Alternative splicing results in multiple transcript variants. But for secondary antibody, I used 1:5000. Western Blot Analysis for COX-1 and GAPDH Proteins The protein level of COX-1 was assessed by Western blot analysis using polyclonal antibodies which were. Many pseudogenes similar to this locus are present in the human genome. My GAPDH is a rabbit polyclonal antibody, using dilution of 1:20000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Western blot analysis of extracts of various cell lines, using GAPDH antibody (A10868). Also, this protein contains a peptide that has antimicrobial activity against E. The stable and ubiquitous expression of GAPDH also make it a suitable loading control for many experiments. The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. GAPDH (36 kDa) is integral for glycolysis and plays many roles in nuclear function such as transcription regulation and apoptosis. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. Values are mean standard error of the mean. Anti-GAPDH Antibody (0411) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry. Protein Kinase C Signaling Pathway Pathway Western blots were normalised to GAPDH and densitometric analysis was performed using image processing software. Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining.Cell Intrinsic Innate Immunity Signaling Pathway.Jak-Stat-IL-6 Receptor Signaling Pathway.Epigenetic writers and erasers of core Histones.
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